NSWBI Logo
Annual Report
Nova Scotia Wild Blueberry Institute
2000 ~ 2001
Date Published: March 1, 2001

  1. Introduction
  2. Board Members 2001-2002
  3. Field Station Summary
  4. Wild Blueberry Research Projects
  5. Manager's Report
  6. Research and Development Fund
  7. Nova Scotia Wild Blueberry Institute Financial Reports
  8. Wild Blueberry Canadian Foundation for Innovation Project

    1.   INTRODUCTION

In 1983, the Honourable Roger Bacon, Minister of Agriculture and Marketing, appointed the first Board of Directors of the Nova Scotia Wild Blueberry Institute (NSWBI). Since its creation, the Nova Scotia Wild Blueberry Institute has been able to achieve a steady increase of research involvement each year, and continues to make contributions to many aspects of the Wild Blueberry Industry.

The Nova Scotia Wild Blueberry Institute has the responsibility to act as a coordinating body for grower oriented research, and provide assistance to the Wild Blueberry Industry through research, demonstration projects, educational programming, and technology transfer.

It is managed by a Board of Directors, and is comprised of representatives from four cooperating agencies:
I. Nova Scotia Department of Agriculture and Fisheries
II. Wild Blueberry Producers' Association of Nova Scotia
III. Nova Scotia Agricultural College
IV. Agriculture and Agri-Food Canada

The Directors and Chair are appointed by the Minister, Nova Scotia Department of Agriculture and Fisheries. Under the direction of the NSWBI Board, the Manager performs the duties at the Field Station, Debert Air Industrial Park.

The elimination of the Production Technology Branch, Nova Scotia Department of Agriculture and Marketing has resulted in changes to NSWBI staff and Board Members. Andrew King, NSWBI Field Manager; Lorne Crozier, NSWBI Secretary/Entomologist; and Dale McIsaac, Blueberry Specialist; are no longer part of the NSWBI team. We acknowledge their valuable contributions and years of service to the Nova Scotia Wild Blueberry Institute. In the interim, Sara Reid has been appointed as Field Manager and Secretary by the Board.

The 2000/01 season at the Nova Scotia Wild Blueberry Institute (NSWBI) was very active and productive. This year's annual report for the Nova Scotia Wild Blueberry Institute (NSWBI) provides an overview of the activities, projects, and programs that the NSWBI was involved in over the past year. Under the management of its appointed Board Members, all objectives were successfully achieved.

The Nova Scotia Wild Blueberry Institute plans to continue their growth with research involvement in the future and provide the Wild Blueberry Industry with up to date technology transfer of the research conducted at the Field Station.

2.   BOARD MEMBERS

The Nova Scotia Wild Blueberry Institute (NSWBI) is managed by a Board of Directors. The Board is comprised of representatives from the blueberry industry, the Nova Scotia Agricultural College, and the Federal and Provincial Departments of Agriculture.

David Sangster, P.Ag. NSWBI Chairman,
Nova Scotia Department of Agriculture and Fisheries,
Truro
Sara Reid Interim NSWBI Manager and Secretary,
Nova Scotia Department of Agriculture and Fisheries,
Truro
Bruce Mowatt Producer,
Wild Blueberry Producers' Association of Nova Scotia
Keith Crowe Producer,
Wild Blueberry Producers' Association of Nova Scotia
Colleen Cameron Producer,
Wild Blueberry Producers' Association of Nova Scotia
Klaus Jensen, PhD Weed Scientist,
Agriculture and Agri-Food Canada,
Kentville
Kenna MacKenzie, PhD Entomologist,
Agriculture and Agri-Food Canada,
Kentville
David Percival, PhD Wild Blueberry Research Chair,
Nova Scotia Agricultural College,
Truro
Bruce Gray, PhD (Acting) Vice-Principal Academic,
Nova Scotia Agricultural College,
Truro
Peter Swinkels, P.Ag., P.Eng. Market Development Officer,
Nova Scotia Department of Agriculture and Fisheries,
Truro

3.   FIELD STATION SUMMARY
The Nova Scotia Wild Blueberry Institute was very active and productive, during the 2000/01 season. At the Field Station, many new projects were introduced and the long-term trials proceeded. All projects, both new and ongoing, received staff support such as sample taking, lab analysis, plot maintenance, and hand-harvesting.

The Field Manager and summer student were involved in many activities this past season. Such activities included: the provision of support to the researchers and their projects; conducting field station tours and student labs; provision of field sites for new and ongoing trials; and the general maintenance of Institute buildings, demonstration areas and research plots.

The WBPANS Annual Field Day was held this year on June 3, 2000 in Westchester, Cumberland County. NSWBI staff were actively involved in the preparation and organization of this event.

The Nova Scotia Institute of Agrologists were given a NSWBI field tour on October 4, 2000. There were a large number of professionals who participated in this event. At the beginning of the tour opening remarks were given by David Sangster, Chairman, and Sara Reid, Interim Manager. Research presentations were given by Dr. David Percival, and Dr. Leonard Eaton.

The official sod turning ceremony for the new research facility was held on site November 17, 2000 at the Field Station. Official members from the Nova Scotia Wild Blueberry Institute, Nova Scotia Agricultural College, and Wild Blueberry Producers' Association of Nova Scotia were present to give a short presentation and turn the sod. The event resulted in a good turn out of growers, private industry, press, and government personnel. The new building is scheduled to be fully constructed by September, 2001.

The "Wild Blueberry Research and Extension Initiative" project was implemented this year by the NSWBI, in collaboration with Agri-Futures Nova Scotia and the Wild Blueberry Producers' Association of Nova Scotia. This project involved the implementation and coordination of seven new research and extension projects. The successful candidate was Sara Reid. Many other NSWBI and WBPANS activities were completed through this project from March 2000 to March 2001.

4.   WILD BLUEBERRY RESEARCH PROJECTS
The wild blueberry crop is very unique, as it involves managing native stands of plants. Research has become more focussed over the past few years on how to better manage the ‘wild' stands, to understand nutrient dynamics, and the basic biology of the plant. It is a vital part to the Wild Blueberry Industry as it allows for new management techniques to develop and help maintain the viability of the crop.

The Nova Scotia Wild Blueberry Institute is involved in a wide variety of research projects, both on and off site. Each project and their activities conducted in the 2000/01 season, are briefly described on the following pages. For further information and clarification arising from a developed interest in a particular project, please contact the project's leader.

1.   

Dr. Leonard J. Eaton

Oxford Frozen Foods Ltd. Research Professor,
Dept. of Environmental Sciences, NSAC, Truro, NS

1.1   TWO VERSUS THREE YEAR MANAGEMENT OF WILD BLUEBERRY PRODUCTION
Dr. Leonard J. Eaton, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, N.S.
Collaborators: Andrew King, NSWBI; Doug Wyllie, C.L. Stonehouse Enterprises Ltd.

This 12-year study was initiated in 1989 at Debert and Mount Thom to assess the effects of second cropping (a three year management process) on wild blueberry yields over time, and to compare it with the regular two year management system. This was done over two complete cycles that would allow direct comparison of first and second crop yields in two of the 12 years (1994 and 2000). We also assessed the differing effects of burning versus mowing and fertilizer applications versus no fertilizer. The final yields from this 12 year study were obtained in August 2000. Greater yields were realized at both experimental sites in the three year management process (second cropping) than with the regular management practice of biennial cropping during the first six year period, and with the total yields at the end of 12 years. Yields in the two systems were similar, however, at both sites during the second phase of the study. Yields in fertilizer plots were higher than those in unfertilized plots at both sites throughout the study, except during the first six years at Debert. Pruning had no effect on yields at either site, except at Debert at the end of the first 6 year cycle. Preliminary estimates of net incomes (income - management costs) suggest that the returns from both management systems were similar over the 12 years at Debert, but not at Mt. Thom, where the three year system resulted in a better total income, but not net income, over 12 years. Regular fertilizer applications over the 12 years contributed to higher total and net returns at both sites compared to the unfertilized controls. There were no differences in incomes between the two pruning systems, even though burning is more expensive than mowing. The results of this study suggest the management process of second cropping can be a viable alternative to the present biennial management process of cropping every second year, when used with regular fertilizer applications.

.2    EFFECTS OF INCREASING RATES OF PHOSPHORUS FERTILIZER ON WILD BLUEBERRY PLANTS AND SOILS.
Dr. Leonard J. Eaton, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, N.S.
Collaborators: Kevin Sanderson, AAFC Charlottetown; Dr. Glenn Stratton, Dr Phil Warman, Ryan Ring, Dept Environmental Sciences, NSAC

Initiated in 1992 at NSWBI, Debert, and at two separate sites in Prince Edward Island, this long term study was designed to assess the effects of increasing rates of a phosphorus fertilizer on wild blueberry plant growth and production, as well as on soil and plant tissue levels of P and other nutrients. Phosphorus fertilizer was applied in 1992, 1994 and 1996 at rates of 0 to 92 kg P2O5 per hectare at all three sites, in replicated sets of plots. The studies at the two PEI sites were completed in 1997, whereas the study at Debert was continued with fertilizer applications in 1998, and further monitoring of soil and tissue nutrient levels and plant growth and production at least through 2001. To date, the results indicate that repeated applications of increased amounts of phosphorus result in significantly greater amounts of extractable P in the soil over time, compared to unfertilized controls. Smaller increases in P levels in leaf and rhizome tissues of blueberry plants were also observed, but the relationships between soil and leaf tissue P were poor. There were no consistent differences among treatments with respect to stem length, buds and blossoms per stem, or yields, throughout the study. The results of this study suggest that repeated applications of a phosphorus fertilizer increase P levels in soil and plants at different rates, but do not stimulate plant growth and fruit yields.

Ryan Ring, as part of the requirements for his M.Sc. Degree, is studying the efficiency of several soil extraction methods for predicting the availability of soil phosphorus to wild blueberry plants at several levels of soil P. This study is being used as one of his experimental sites.

1.3   USE OF COMPOST AND OTHER MULCHES ON WILD BLUEBERRIES. Dr. Leonard J. Eaton, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, N.S.
Collaborators: Andrew King, NSWBI; Dr. Peter Hicklenton, AAFC, Kentville.

Four types of mulching material, sawdust, municipal solid waste compost, sand/soil and landscape bark mulch were applied to replicated plots at two sites on the NSWBI property at Debert in September 1997. Plot sizes are 1 x 1 m, with 4 replications in the select clone area and 2 in the newly developed area across the road. These plots are monitored through a series of photographs over time, with which we hope to assess spreading patterns associated with the different mulches. More mulching material was added as necessary in the autumn of 2000.

The objective of this long term study is to evaluate the effects of gypsum applied with and without fertilizer on nutrient status and yield of wild blueberries in Prince Edward Island and Nova Scotia. Replicated plots with four treatments (1: control; 2: gypsum @ 4 tonne/ha; 3: 10-10-10 @ 300 kg/ha and 4: gypsum @ 4 tonne/ha + 10-10-10 @ 300 kg/ha) were set up in two site in Prince Edward Island and in three sites in Nova Scotia, during May 1998. Soil and leaf tissue nutrient levels were assessed at tip die back (usually July) in each year. Plant growth and yield data were assessed in the crop year (1999, 2001). The treatments affected levels of soil Ca, S and pH at the three Nova Scotia sites in 1998, and at three of the 5 sites in 1998. Only S levels in leaf tissue were affected by treatment, and demonstrated differences in both years. At the end of the first crop cycle (1999), plant growth and production appeared to be unaffected by treatments, except for buds per stem (2 of 5 sites) and stem length(1 site). We are convinced that this study should be continued for at least one more production cycle.

1.5 MOSS IN WILD BLUEBERRY FIELDS.
Dr. Leonard J. Eaton, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, N.S.
Collaborators: Katriona MacNeil, NSAC; Doug Wyllie, C.L. Stonehouse Enterprises; Morris Lloy.

This study was initiated in 1999 to determine the extent of moss infestation in a number of commercial blueberry fields, to understand the nature of moss growth and spread within commercial blueberry fields, and to begin the search for methods of prevention and control. As part of the study, we mapped the distribution of moss in the mow plot at the NSWBI Field Station, Debert. This is the only part of the site, except for the three year management plots, in which we could find consistent amounts of Polytrichum spp., the type of moss most common in wild blueberry fields. Preliminary data suggest that mosses are beginning to move into commercial fields as a result of tolerance to Velpar, possibly due to lower herbicide rates in recent years. It appears that the mosses do not adversely affect wild blueberry plant growth and production until the moss plants reach 100 % cover. At this point, the numbers of blueberry stems per unit of area are significantly reduced compared to stem numbers in areas where moss is absent. Other aspects of moss - wild blueberry interactions will be reported by Katriona MacNeil, who is studying the problem as part of the requirements for her B.Sc. Degree. Her work was supported by an NSERC Summer Research Fellowship.

2.   

Ryan Ring, Graduate Student
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

2.1   A Comparison of Five Extraction Methods for Determining Available Soil Phosphorus in Nova Scotia Blueberry Soils.
R. A. Ring, P. R. Warman, G. W. Stratton, L. J. Eaton, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, NS.
Collaborators: Nova Scotia Wild Blueberry Institute, Bragg Lumber Company, WBPANS, NSERC, Chesley Walsh, and Jim Burgess.

A project was initiated to determine the best predictor of plant available phosphorus, for the Nova Scotia lowbush blueberry, from the following extractants: Mehlich 1, Mehlich 3, Bray 1, Modified Morgan and the Anion Exchange Membrane (AEM). The project was initiated in 1999 with three newly established sites; two loamy soils located in South Branch (South Branch and White Fields), and one sandy loam soil in Debert. Two additional sites, both sandy loams, one located in Parrsboro and the other at the Wild Blueberry Institute are also being used. Soil samples were taken in the spring and at tip dieback or harvest (late summer) from all sites during the 1999 and 2000 growing seasons. Tissue samples were obtained, with the late summer soil samples, to correlate soil phosphorus with the phosphorus present in the tissue. All data obtained to date was statistically analyzed with the SAS System software package. Results from the 1999 and 2000 seasons indicate that the complexity with which the blueberry plant takes in P, affects the ability of the extractants to predict leaf P concentrations. It would appear that at low levels of extractable P (<10 mg extractable P kg-1 soil) none of the five extractants can accurately predict leaf P concentrations. However, the White Field site did have significant correlations ® > 0.60) for all five extractants, due in large part, to the high rates of applied P which resulted in levels of P higher than those obtained at the other sites. The remaining sites had levels of P which were too low to accurately predict the P uptake of the plant. Further work on the uptake of P by the blueberry plant under conditions of low and high levels of extractable P is required.

3.   

Dr. David Percival
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

3.1    NITROGEN FORMULATION INFLUENCES PLANT NUTRITION AND YIELD COMPONENTS OF LOWBUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.)
D.C. Percival1, and J.P. Privé 2. Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia1. Agriculture and Agri-Food Canada, Bouctouche, New Brunswick2.
Collaborators: Aubrey Atkinson, WBPANS, Nova Scotia Agricultural College, Bragg Lumber Company, Nova Scotia Wild Blueberry Institute.

A study examining the influence of nitrogen formulation and application date was conducted at the Nova Scotia Wild Blueberry Institute and at a commercial field at West Brook N.S. from 1997 to 2000. Nitrogen applications consisted of applying 25 kg·ha-1 N in the autumn following the cropping year of production (September and October), or during the spring (May) of the vegetative year of production. The nitrogen sources consisted of ammonium nitrate (AN), ammonium sulphate (AS), or sulphur coated urea (SCU), and were applied in a 7-14-7 mixture (N:P2O5:K2O). No deleterious effects of autumn nitrogen applications on winter hardiness were observed in this study. Although fertilizer application, application date, and nitrogen formulation had a significant impact on leaf tissue nitrogen content, these differences were less than 7%. Fertilizer applications had a beneficial effect on yield at all sites examined with yield increases of 23, 27, and 25% being observed at the NSWBI (1999), NSWBI (2000), and West Brook sites respectively. These yield increases were partially due to increased berry numbers per stem, with 7.6, 82, and 10% more berries per stem being present than the control at the NSWBI (1999), NSWBI (2000) and West Brook sites respectively. Nitrogen formulation had a significant influence on yield at all 3 sites with the fruit set and yield of the AN treatments being lower than the AS and SCU treatments. Therefore, the results from this experiment indicate that nitrogen formulation can influence the yield potential of lowbush blueberries, and must be considered in fertility management practices.

4.   

Allison Murray, Fourth-Year Student
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

4.1 DROUGHT STRESS SIGNALING AND SUBSEQUENT GROWTH DYNAMICS OF THE WILD LOWBUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.) Allison Murray, David Percival, Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, NS. Collaborators:WBPANS, Nova Scotia Agricultural College, Bragg Lumber Company, and the Nova Scotia Wild Blueberry Institute.

This research project was completed from April to August at the Nova Scotia Wild Blueberry Institute (NSWBI), in Debert Nova Scotia. There were ten plots in total, each plot was divided in half. The treatments consisted of plots with shelter with a cut border, no shelter with cut border, shelter with uncut border, and no shelter with uncut border. The objectives of the research were to examine the contribution of lateral movement of water on (1) leaf fluorescence, (2) leaf gas exchange, (3) growth and development dynamics, and (4) yield of wild lowbush blueberries. To monitor the soil moisture throughout the summer, soil moisture probes were placed in each half of the plots. The examination of these objectives included taking leaf fluorescence measurements with a fluorescence meter and photosynthesis measurements to give an indication of any internal effects to the blueberry plant. Sap flow measurements on the blueberry plant were collected three times throughout the summer. Sap flow was determined to monitor water flow and lateral water movement (rhizomes) in the blueberry plants. To assess growth and development, vegetative samples were collected from all plots. Stem length, floral zone length, number of set fruit, vegetative and flowering node numbers and berry weight per stem were collected then analyzed. At the end of August, harvestable yield was hand-raked and total plot weights were recorded to later determine average berry weight. Due to below average rainfall in Nova Scotia during the crucial wild blueberry growing season the past few years, information on the effects of drought stress signaling between plants and growth are important to blueberry producers. Information obtained in this study may provide industry with insight toward yield, growth and development and how they are affected under drought stress.

5.   

Bonna Jordan, Graduate Student
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

5.1 IMPACT OF LIVING MULCHES ON THE ENVIRONMENT AND GROWTH DYNAMICS OF THE LOWBUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.)
Bonna Jordan1, Dr. David Percival1, Dr. Gordon Brewster1, Gary Patterson2. Department of Environmental Sciences, Nova Scotia Agricultural College, Truro, N.S.1 Agriculture and Agri-Food Canada, Truro, N.S. 2
Collaborators: WBPANS, Nova Scotia Wild Blueberry Institute, Bragg Lumber Company, and Bruce Mowatt.

With the increase in the use of herbicides such as hexazinone (Velpar) bare spots have become a common occurrence in wild blueberry fields. With no supportive vegetative cover, these bare spots are susceptible to soil erosion and their microclimate conditions are not very favourable for blueberry growth. Soil erosion reduces soil quality and the coverage of lowbush blueberries. It has been proposed that this problem can be reduced by using living mulches. The objectives of this study were to examine the establishment characteristics of the living mulches, the changes in the physical environment that occur as a result of the living mulches, and the subsequent effects on the growth dynamics of the lowbush blueberry. Studies were conducted at two commercial fields in Upper Stewiacke, Nova Scotia. The living mulch treatments for this project were recommended from a single- species vegetation management study in Debert, Nova Scotia. The first field was established in 1998 with the treatments consisting of (1) a control, (2) creeping red fescue and perennial ryegrass and (3) birdsfoot trefoil and tall fescue. Treatments for the second field established in 1999 were (1) a control, (2) birdsfoot trefoil, perennial ryegrass, tall fescue, and (3) nordic hard fescue, sheep's fescue, creeping red fescue and perennial ryegrass. Both fields were organized in a randomized block experimental design and were in sprout year in 1998 and 2000. With two years of data, results indicate no detrimental interactions occurring between the living mulches and lowbush blueberry and an improvement in soil physical conditions (i.e. hydraulic conductivity and infiltration rate). With these improvements lowbush blueberries have an increased potential of spreading into these once bare areas decreasing soil erosion and improving blueberry coverage.

6.   

Lindsay Hainstock, Graduate Student
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

6.1   GETTING BACK TO BASICS: CARBON ASSIMILATION AND ALLOCATION IN WILD BLUEBERRY PLANTS
Lindsay Hainstock 1, David Percival1, J.P. Privé 2, and Nancy Crowe 1. Dept. of Environmental Sciences, Nova Scotia Agriculture College, Truro, N.S.1 Atlantic Food & Horticulture Research Centre, Bouctouche N.B.2
Collaborators: WBPANS, Nova Scotia Wild Blueberry Institute, Bragg Lumber Company, and NSERC.

The objectives of this experiment were to measure the assimilation rate of carbon and how it is allocated throughout the lowbush blueberry plant for both the sprout and crop 1999-2000 growing season at the Nova Scotia Wild Blueberry Institute. Carbon Assimilation: Photosynthetic readings were measured weekly on the stems of blueberry plants in both the crop and sprout stage of production from May to October. The stems were then removed and brought back to the lab where the leaf areas were measured and the photosynthetic pigments extracted for measurement. On the same day, the fluorescence of individual leaves was measured under natural light and under dark-adaptation. Carbon Allocation: Whole plant samples were removed every 2-3 weeks from April to November. The plant were brought back to the Nova Scotia Agriculture College where they were cleaned, dried, and separated into stems, roots, rhizomes, and berries. The dry weights were recorded and the samples ground for carbohydrate analysis. Data is presently being analyzed.

7.   

Ekaterina Jeliazkova
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

7.1    INFLUENCE OF DROUGHT STRESS ON MYCORRHIZAL ASSOCIATIONS IN LOWBUSH BLUEBERRY.
Ekaterina Jeliazkova, and David Percival. Dept. of Environmental Sciences, Nova Scotia Agricultural College, Truro, NS.
Collaborators: Bragg Lumber Company, Nova Scotia Wild Blueberry Institute, and Wild Blueberry Producers Association of Nova Scotia.

A drought stress study was conducted at the Nova Scotia Wild Blueberry Institute. The study was aimed to determine the impact of drought stress on the mycorrhizal infection levels in lowbush blueberry (Vaccinium angustifolium Ait.) roots. In addition, the spatial allocation of the mycorrhizal associations was assessed. A field experiment with a completely randomized block design was set up. The treatments consisted of control (no water exclusion and naturally rain fed) and drought plots (water exclusion, achieved by using transparent sheds placed at approximately 50 cm above the ground). The soil moisture content in the treatment plots was measured using CS615 soil moisture probes (Campbell Scientific) and the readings recorded hourly by a CR10X Data logger. The study was conducted simultaneously in both, the vegetative and cropping stage of production. Within each stage of production five replications were used per treatment, and within each replication five samples were collected. Each collected sample, a soil core, was 2.5 cm in diameter and 15 cm deep. To assess the spatial allocation of the mycorrhizal fungi in the blueberry roots the soil core samples were divided into upper (0 – 7.5 cm) and lower (7.5 –15 cm) portions. The collected samples were stored in a refrigerator until needed and further processed for mycorrhizal examination as follows. The blueberry roots were separated from the soil by washing with tap water through a stack of sieves until clean. The cleaned roots were cut into segments, approximately 20 mm in length, and prepared for microscopic examination by further clearing, following the procedures reported by Boyer et al. (1982), Kormanik and McGraw (1982), and Brundrett et al. (1996). The cleared root segments were stained using 0.01 % Chlorazol Black E. The root segments, each from 0.075 to 0.1 mm in diameter, were examined for mycorrhizal infection using light microscope. Colonization levels were assessed at X 400 and expressed as percent mycorrhizal infection. Percent infection was calculated by measuring mm of the root segments containing hyphae, divided by the total number of mm per root segment, and multiplied by 100. The mycorrhizal associations were equally distributed in the soil profiles of 0-7.5 cm and 7.5-15 cm depth, with percent infection ranging from 72.4% to 68.9%, respectively. Drought had no effect on the mycorrhizal infection levels with percent infection being 69.1% and 71.2% for the drought and control treatments, respectively. Overall, the results of this research indicated that the percent incidence of the mycorrhizal associations in the wild blueberry roots is relatively high. In addition, the study showed that the mycorrhizal associations were equally distributed in the soil profile of 0 to 15 cm and were not affected by the soil moisture status. During 2001, we plan to expand the research and evaluate the impact of the mycorrhizal associations on nutrient assimilation and allocation and overall growth and development of the wild blueberry.

8.   

Peter Burgess, Graduate Student
Dept. of Environmental Sciences,
Nova Scotia Agricultural College, Truro, NS

8.1    EFFICACY AND CROP PHYTOTOXICITY OF SEVERAL HERBICIDES ON COMMERCIAL WILD BLUEBERRIES VACCINIUM ANGUSTIFOLIUM AIT. AND INTRODUCED LIVING MULCHES.<
Collaborators: Andrew King, NSWBI; and Sara Reid, NSWBI

This study involved two distinct phases. A herbicide screening study was done at the Nova Scotia Wild Blueberry Institute, from which herbicides that showed low phytotoxic effects could be tried on a second phase, an introduced living mulch greenhouse trial at the NSAC. This trial looked at the herbicide effectiveness on the introduced living mulches and also looked at herbicide tolerance. The NSWBI portion of the study involved a section of relatively homogenous, semi-commercial wild blueberry field, used to test the phytotoxic effects of 11 treatments on the blueberry plants. Treatments of a standard, (hexazinone, applied to entire treatment area earlier in spring after burning), imazethapyr, nicosulfuron/rimsulfuron, thifensulfuron-methyl, chlorimuron, Flumetsulam/metolachlor, fluazifop-p-butyl, prosulfuron, rimsulfuron-elim, quizalofop-ethyl and clethodim were applied, on top of the hexazinone application before full emergence. Phytotoxic characteristics were rated for each plot on a 0-9 scale ( 0 = nil affect, 9 = plant death ). Ratings were done at one, four, and eight weeks after application. The greenhouse study was set up as a completely randomized design and involved five different living mulch species (Creeping Red Fescue, Hard Fescue, Sheep Fescue, Perennial Rye Grass, and Birdsfoot Trefoil). Fluazifop-p-butyl, prosulfuron, nicosulfuron/rimsulfuron (Ultim), tribenuron-methyl, hexazinone, and a control (no herbicide application) were applied on each of the five living mulches and replicated five times. In the field trial at the Nova Scotia Wild Blueberry Institute Chlorimuron, imazethapyr and flumetsulam/metolachlor produced mean phytotoxic effects greater than (2.75). This high rate was deemed to be unacceptable for further study at full rate. Fluazifop-p-butyl (Fusillade), prosulfuron (Peak), and nicosulfuron/rimsulfuron (Ultim) showed mean phytotoxicity ratings lower than (2.75) and had desirable action spectrums. The broad spectrum herbicides nicosulfuron/rimsulfuron and hexazinone, showed a very high phytotoxic activity when applied to the introduced living mulches in the greenhouse. However, there was some tolerance with creeping red fescue and sheep fescue when fluazifop-p-butyl and tribenuron-methyl were applied. Further study into varying rates of different herbicides should be done to develop an integrated vegetation management plan. Yield data was collected on the field site in August of 2000. The treatment site was in a recently levelled area and as a result harvesting efficiency was reduced, due to exposed roots and low growing plants. Decreased harvesting efficiency combined with natural variation and less than 60% plant coverage throughout the treatment area resulted in no significant treatment differences with regards to yield at this location.

9.1 Dr. Sonia Gaul
Agriculture and Agri-Food Canada, Kentville, NS

9.1    IMPROVED IPM OF THE BLUEBERRY FRUIT FLY.
Dr. Sonia Gaul, Agriculture and Agri-Food Canada, Atlantic Food and Horticulture Research Centre, 32 Main Street, Kentville, N.S.
Collaborators: Sara Reid, Amanda Hampton, NSWBI

The interception of the blueberry fruit fly, Rhagoletis mendax Curran, before the female adult lays eggs in the developing fruit is the basis of the insect control strategy for this pest of lowbush blueberries. Studies in 2000 have investigated the influence of trap spacing on the interception of adults of the blueberry fruit fly in lowbush blueberry fields at the NSBI site. There was no change in seasonal trap captures from July 17, 2000 to August 18, 2000 with decreased distance between traps, from 2.5 m to 10 m. Average total seasonal trap captures were 25 at 2.5 m, 14 at 3.75 m, 17 at 5 m, 15 at 7.5 m and 15 at 10 m.

5.   MANAGER'S REPORT
Sara Reid, Interim Field Manager

There were a lot of valuable results produced at the conclusion of the 2000/01 season. There were many research projects, both long and short-term that were concluded, as well as, seven new research and extension projects that were implemented.

The "Wild Blueberry Research and Extension Initiative" allowed for a lot of well-needed industry projects to be completed. The projects are as follows:

  • NSWBI Business Plan
  • Weed Demonstration Garden
  • Farm Management Analysis Pilot Project
  • Slide and Photo Gallery
  • Five new factsheets completed for the Wild Blueberry Handbook
  • Industry Webpages: NSWBI and WBPANS
  • WBPANS Annual Meeting Booklet

The staff at the NSWBI Field Station this past season were Sara Reid and Amanda Hampton. Sara was funded by Agri-Futures Nova Scotia for the entire year, while Amanda was funded by the Wild Blueberry Producers' Association of Nova Scotia for the summer months and was shared with the NSDAM Insect Survey.

Each year there are a new set of research priorities produced by the industry. As the research priorities change, the topics covered at the NSWBI reflect the industry needs. In 2000/01, the following topics were covered:

  • Two versus Three-Year Management
  • Leaf Gas Exchange/Photosynthesis
  • Carbon Assimilation Study
  • Drought/Irrigation Study
  • Mycorrhizal Association
  • Nitrogen Fertilizer Experiment
  • Fall versus Spring Fertilization
  • Effects of Fertilizer, Lime and Gypsum
  • Effects of Phosphorus
  • Soil Phosphorus Extraction
  • Compost Study
  • Vegetation Management
  • Herbicide Effect on Native Plants and Living Mulches
  • Fruit Fly Trapping Study
  • Moss Study
  • Weed Trials
  • Erosion Study

Demonstration projects included:

  • Long Term Pruning Trials
  • Weed Collection
  • Select Clones
  • Equipment
  • Hybrid Seedling Plots

The back field was harvested in 2000 and averaged 2,447 lbs/acre (14,684 lbs/6 acres). A newly developed site across the road was also harvested (patch picked) and averaged 647 lbs/acre (2,587 lbs/ 4acres).

Visitors on site included growers, researchers, students, and interest groups.

The new land acquired by the Nova Scotia Wild Blueberry Institute has developed considerably since it was cleared in 1994. Plant growth and spread seem to be very rapid on this land. The left hand part of this field was harvested for the first time in 1998, and the right hand side for the first time in 1999. Both pieces were stumped and levelled in 2000.

6.   RESEARCH AND DEVELOPMENT FUND From 1992 to 2000, numerous projects received funding from the Nova Scotia Wild Blueberry Institute Research and Development Fund, totalling $102,400.

7.   NOVA SCOTIA BLUEBERRY INSTITUTE FINANCIAL REPORTS

1.0      

Nova Scotia Blueberry Institute
Financial Report
Operating Fund

As of March 31, 2000 to January 31, 2001

Balance: March 31, 2000 (Year End) $ 47,964.41
Revenue (2000):
NS Dept of Agriculture & Fisheries (Grant) $20,000.00
WBPANS (Grant) 4,000.00
Sale of Blueberry Crop 8,404.48
Sale of Lumber (Land Clearing) 4,000.00
WBPANS Contribution (Agri-Futures) 3,500.00
Agri-Futures Nova Scotia 18,000.00
Total Revenue: $ 57,904.48
Expenses:
Casual Employee Wages and Fringe Benefits 19,981.45
CFI Project* 10,000.00
Agri-Futures Contribution* 3,650.00
General Operating 9,754.97
Custom Work (services) 6,663.08
Telephone 442.00
Electricity 715.74
Total Expenses: ( 51,207.24)
Balance: January 31, 2001
$ 54,661.65
** These entries are officially committed by the
Nova Scotia Wild Blueberry Institiute, but not yet expended **

1.1      

Nova Scotia Blueberry Institute
Financial Report
Research and Development Fund

As of March 31, 2000 to January 31, 2001

Balance: March 31, 2000 $ 7,800.00
Expenses (2000):
Total Expenses: ($ 0.00)
Balance: January 31, 2001 $7,800.00


8.   WILD BLUEBERRY CANADIAN FOUNDATION FOR INNOVATION PROJECT

The CFI project is an integrated systems approach to improving wild blueberry physiology, protection, and productivity. It is a very unique and innovative way to increase the sustainability of the wild blueberry industry. Highly qualified personnel will become trained in the latest trends in wild blueberry research and industry concerns. The wild blueberry industry provides the largest value to the province's economy out of all fruit crops, therefore this initiative is of regional importance.

A new research facility will be constructed at the Field Station, Debert Air Industrial Park, as a component of this project. The desired completion date of this facility is September, 2001. This building will become a headquarters for the wild blueberry industry. It will provide office and lab facilities for participants of the NSWBI, as well as, office space for the Wild Blueberry Producers' Association of Nova Scotia. At the WBPANS Annual General Meeting in November, 2000, Dave Sangster, Chair, NSWBI gave a presentation to inform the industry on the latest developments of this new research facility. ( A detailed plan of the building's structure is located on the next page.)

The original facilities located at the Field Station were established in 1983 and need to be replaced. The new research facility will enhance the research capability with the completion of this project.

The Canadian Foundation for Innovation and the Nova Scotia Council for Higher Education provided matching industry funds for this multi-stakeholder project. The industries' financial contributions were made available by the Nova Scotia Wild Blueberry Institute and the Wild Blueberry Producers' Association of Nova Scotia.

Another component of this project is the purchase of new equipment to compliment the research being conducted at the Field Station. The equipment will include a root temperature system, spectrophotometer and cell positioner, microscopic and image analysis gear, and environmental monitoring stations. This equipment will allow for the quality of the research conducted at the NSWBI, NSAC, AAFC, and other institutions to be improved.

Wild blueberry research and technology transfer will be increased with the implementation of this project. The Nova Scotia Wild Blueberry Institute is pleased to be a partner in this new research facility.

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For further information, please contact Wild Blueberry Network Information Centre (WBNIC@nsac.ca).